Trizzino;1102436 wrote: If you hold the button down when you are on C2 test vial it starts a countdown from 3 minutes allowing the Phosphate reagent to mix and bubbles to settle.
To expand on this... what I've found helps with the Ultra Low Phos is before I start I put a clock on my phone that shows seconds. Then do it in this order with one cuvette.
1. I prepare the reagent packet.
2. Put 10ml in the vial.
3. Start the checker, at C1 I put the vial in and press the button.
4. Once the unit shows C2 I note the time and take the vial out.
5. Add as much of the reagent to the vial as I can get out of the packet then start shaking it as I watch the time.
6. At about 1:50 elapsed time (1 minute 50 seconds) I hold in the button to start the 3 min timer while still shaking the vial.
7. Once the reagent looks mixed well I let it settle for a few seconds then start inverting it slowly while rotating the vial. This clears the small bubbles.
8. Wipe the vial down good making sure the outside is clean. Then insert it into the unit to take the reading.
At the last step I usually have a little more than a minute left and the results are very repeatable, within 3-5ppb. This is the only Hanna test that you have to watch the time and doing it this way also helps me to be sure I don't miss the reading. I'm sure we've all put the vial in first then started the timer, set the thing down and walked away to do something else. Only to return to go :doh: as you look at a blank screen.
The key to Hanna, and all other tests for that matter, is starting with a clean vial. I keep a Tupperware container with RODI water in it that I use to rinse the vials as soon as the test is done then set them in a drying rack to use again. I can usually get 3 to 4 tests per vial before I need to give them a good cleaning.