I never attempt to maintain bacteria in a hospital tank, as it's really pointless. The med's often destroy bacteria anyway, and in the case of quinine it's certain to.
I also use a bare bottom as substrate is of no benefit and may adsorb some med's. Many variables here so I prefer to eliminate them, if possible.
Daily partial water changes keep ammonia down, water parameters more constant, and allow for constant dosing/stable medication levels.
I also like to use large pieces of PVC to provide inert structure for the fish to hide around, as well as low light. These help to reduce stress.
So,
- a plain glass box (tank, Rubbermaid containers, plastic sinks, etc. all work. Just don't ever use for food prep after, due to the med's used)
- fish to be treated
- some large PVC pieces for inert structure
- daily dosing, if possible (refer to directions)
- daily partial water changes to export nutrients (mostly ammonia)
- low light/low stress in a quiet area